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KMID : 0881720090240040312
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Journal of Food Hygiene and Safety
2009 Volume.24 No. 4 p.312 ~ p.317
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Establishing Test Method of Sporicidal activity of Commercial Sterilants
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Kim Hyung-Il
Jeon Dae-Hoon
Yoon Hae-Jung
Kwak In-Shin
Eom Mi-Ok
Sung Jun-Hyun
Park Na-Young
Won Sun-Ah
Bae Seo-Young
Lee Young-Ja
Kim So-Hee
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Abstract
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Usually, bacterial spores are hundreds or thousands of times more resistant to chemical sanitizers than are vegetable bacteria. Consequently, it is hard to assess whether a commercial sterilant, containing hydrogen peroxide and peracetic acid as ingredients, has or does not have sporicidal activity under certain conditions using the National Standard Test Method for assessing bactericidal activity against Escherichia coli and Staphylococcus aureus. Hence we established alternative the standard test method and requirements to determine whether they are effective in showing at least reduction of 103 in the number of Bacillus subtilis ATCC 6633 spores under the required test condition for evaluation of sporicidal activity including verification methodology. This standardized method has proved to be suitable for evaluating effectiveness of commercial sterilants and could be used as Standardization Test Method for assessing sporicidal activity.
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KEYWORD
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commercial sterilants, hydrogen peroxide, peroxyacetic acid, sporicidal activity
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